We discuss step-by-step methods for enzymatic bioconjugation and enzyme-catalyzed bioconjugation with tyrosinase, sortase, and glutamate racemase.
We discuss step by step methods for n-terminal bioconjugation of proteins including N-terminal methylation, biotinylation, acetylation, and labeling.
We describe how to use palladium in bioconjugation of proteins and DNA by providing step-by-step conjugation instructions and discussing applications.
We discuss azide bioconjugation reactions including step-by-step instructions for performing click chemistry with and without copper.
We discuss methods for the bioconjugation of nanoparticles. We discuss different nanoparticles, functional groups, and methods for attaching to functional…
We discuss how to choose the right bifunctional crosslinkers for conjugation reactions including homobifunctional, heterobifunctional, and photoreactive linkers.
We discuss common bioconjugation problems related to protein, DNA, antibody, and enzyme bioconjugates and their solutions.
We discuss different tryptophan bioconjugation methods including ABNO, UV crosslinking, electrochemical and photochemical reactions, and site-selective reactions.
We discuss different DNA bioconjugation methods and applications. We discuss amine, thiol, and intercalation and site-specific bioconjugation.
We discuss different methods for bioconjugation of pegylated nanoparticles including thiol and amine reactions and enzymatic conjugation reactions