We discuss step-by-step methods for enzymatic bioconjugation and enzyme-catalyzed bioconjugation with tyrosinase, sortase, and glutamate racemase.
We discuss step by step methods for n-terminal bioconjugation of proteins including N-terminal methylation, biotinylation, acetylation, and labeling.
We discuss azide bioconjugation reactions including step-by-step instructions for performing click chemistry with and without copper.
We discuss how to choose the right bifunctional crosslinkers for conjugation reactions including homobifunctional, heterobifunctional, and photoreactive linkers.
We discuss different tryptophan bioconjugation methods including ABNO, UV crosslinking, electrochemical and photochemical reactions, and site-selective reactions.
We discuss different methods for bioconjugation of pegylated nanoparticles including thiol and amine reactions and enzymatic conjugation reactions
We discuss different methods for the bioconjugation of carbon nanotubes including amine, aldehyde and carboxyl based methods.
We discuss orthogonal bioconjugation techniques and methods such as SPAAC & tetrazine ligation, as well as applications of bioorthogonal chemistry.
We discuss different methods for bioconjugation to surfaces of polymers, DNA, and proteins such as tyrosine and lysine bioconjugation.