We discuss step-by-step methods for enzymatic bioconjugation and enzyme-catalyzed bioconjugation with tyrosinase, sortase, and glutamate racemase.
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We discuss step by step methods for n-terminal bioconjugation of proteins including N-terminal methylation, biotinylation, acetylation, and labeling.
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We discuss azide bioconjugation reactions including step-by-step instructions for performing click chemistry with and without copper.
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We discuss how to choose the right bifunctional crosslinkers for conjugation reactions including homobifunctional, heterobifunctional, and photoreactive linkers.
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We discuss different tryptophan bioconjugation methods including ABNO, UV crosslinking, electrochemical and photochemical reactions, and site-selective reactions.
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We discuss different methods for bioconjugation of pegylated nanoparticles including thiol and amine reactions and enzymatic conjugation reactions
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We discuss different methods for the bioconjugation of carbon nanotubes including amine, aldehyde and carboxyl based methods.
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We discuss orthogonal bioconjugation techniques and methods such as SPAAC & tetrazine ligation, as well as applications of bioorthogonal chemistry.
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We discuss different methods for bioconjugation to surfaces of polymers, DNA, and proteins such as tyrosine and lysine bioconjugation.
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