We discuss different NHS ester bioconjugation protocols for reactions with amines and hydroxyls. We also discuss hydrolysis and the use of DMSO.
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We discuss step-by-step bioconjugation methods for radiopharmaceutical chemistry including chelation, pegylation, and bioorthogonal reactions.
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We discuss different cysteine bioconjugation techniques, applications, and step by step protocols including strategies which use palladium catalysts, vinyl heteroarenes, and more.
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We discuss step-by-step methods for enzymatic bioconjugation and enzyme-catalyzed bioconjugation with tyrosinase, sortase, and glutamate racemase.
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We discuss step by step methods for n-terminal bioconjugation of proteins including N-terminal methylation, biotinylation, acetylation, and labeling.
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We describe how to use palladium in bioconjugation of proteins and DNA by providing step-by-step conjugation instructions and discussing applications.
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We discuss azide bioconjugation reactions including step-by-step instructions for performing click chemistry with and without copper.
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We discuss methods for the bioconjugation of nanoparticles. We discuss different nanoparticles, functional groups, and methods for attaching to functional groups.
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We discuss how to choose the right bifunctional crosslinkers for conjugation reactions including homobifunctional, heterobifunctional, and photoreactive linkers.
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We discuss common bioconjugation problems related to protein, DNA, antibody, and enzyme bioconjugates and their solutions.
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